Recombinant Human BIRC5, transcript variant 1, fused with MYC/DDK tag at C-terminal was expressed in HEK293.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
>50 ug/mL as determined by microplate BCA method