Recombinant Human BMP4(Ser293-Arg408)/BMP7(Ser293-His431) was expressed in E. coli.
Predicted N Terminal:
Met (BMP-4) & Ser293 (BMP-7)
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 15-75 ng/mL.
Predicted Molecular Mass: 13.3 (BMP-4), 15.8 (BMP-7) kDa
<0.01 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile 4 mM HCl.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.