Active Recombinant Human BMP7 Protein, Biotinylated

• Cat.No.: BMP7-163H
• Product Overview: Recombinant Human BMP7(Ser293-His431) was expressed in CHO, Biotinylated.

Specification

• Description: Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease. Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain. The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains. Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers. The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains. Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin. BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB. BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial-mesenchymal transition (EMT). In pathological conditions, BMP-7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis, and promotes neuroregeneration following brain ischemia.
• Source: CHO
• Species: Human
• Predicted N Terminal: Ser293
• Form: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 100-600 ng/mL.
• Molecular Mass: 16 kDa (unlabeled)
• Protein length: Ser293-His431
• Endotoxin: <0.010 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE with silver staining
• Notes: Structure / Form: Disulfide-linked homodimer; Biotinylated protein via sugars
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.

Gene Information

• Gene Name: BMP7 bone morphogenetic protein 7 [ Homo sapiens ]
• Official Symbol: BMP7
• Synonyms: BMP7; bone morphogenetic protein 7; OP 1; osteogenic protein 1; BMP-7; OP-1
• Gene ID: 655
• mRNA Refseq: NM_001719
• Protein Refseq: NP_001710
• MIM: 112267
• UniProt ID: P18075