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Home > Products > Recombinant Proteins > Recombinant Human BRAF protein, MYC/DDK-tagged

Recombinant Human BRAF protein, MYC/DDK-tagged

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Cat.No.:
BRAF-22H
Product Overview:
Recombinant Human BRAF fused with MYC/DDK tag at C-terminal was expressed in HEK293.
Description:
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Source:
HEK293
Species:
Human
Tag :
MYC/DDK
Form:
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Bio-activity:
BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37°C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader.
Molecular Mass:
84.3 kDa
Purity:
> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:
>50 ug/mL as determined by microplate BCA method


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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