Active Recombinant Human BRAF protein, MYC/DDK-tagged
Cat.No. : | BRAF-22H |
Product Overview : | Recombinant Human BRAF fused with MYC/DDK tag at C-terminal was expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
Source : | HEK293 |
Species : | Human |
Tag : | MYC/DDK |
Form : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Bio-activity : | BRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37°C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader. |
Molecular Mass : | 84.3 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration : | >50 ug/mL as determined by microplate BCA method |
Gene Name : | BRAF v-raf murine sarcoma viral oncogene homolog B1 [ Homo sapiens ] |
Official Symbol : | BRAF |
Synonyms : | BRAF; v-raf murine sarcoma viral oncogene homolog B1; serine/threonine-protein kinase B-raf; BRAF1; p94; 94 kDa B-raf protein; proto-oncogene B-Raf; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94); NS7; RAFB1; B-RAF1; FLJ95109; MGC126806; MGC138284; |
Gene ID : | 673 |
mRNA Refseq : | NM_004333 |
Protein Refseq : | NP_004324 |
MIM : | 164757 |
UniProt ID : | P15056 |
Chromosome Location : | 7q34 |
Pathway : | ARMS-mediated activation, organism-specific biosystem; Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; |
Function : | ATP binding; MAP kinase kinase kinase activity; metal ion binding; mitogen-activated protein kinase kinase binding; nucleotide binding; protein binding; protein heterodimerization activity; protein kinase activity; protein serine/threonine kinase activity; receptor signaling protein activity; |
Products Types
◆ Recombinant Protein | ||
BRAF-456H | Recombinant Human BRAF Protein, His (Fc)-Avi-tagged | +Inquiry |
BRAF-2782H | Recombinant Human BRAF Protein, His-tagged, BSA Conjugated | +Inquiry |
Braf-441M | Recombinant Mouse Braf Protein, MYC/DDK-tagged | +Inquiry |
BRAF-1081M | Recombinant Mouse BRAF Protein, His (Fc)-Avi-tagged | +Inquiry |
BRAF-2781H | Recombinant Human BRAF Protein, His-tagged, BSA Conjugated | +Inquiry |
◆ Lysates | ||
BRAF-8414HCL | Recombinant Human BRAF 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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