Recombinant Human C1QL3(His19-Glu563) fused with MYC/DDK tag at C-terminal was expressed in HEK293.
C1qTNF proteins constitute a highly conserved family of Acrp30/Adiponectin paralogs that share a modular organization comprising an N-terminal signal peptide, a short variable region, a collagenous domain and a C-terminal globular domain. C1qTNF proteins are predicted to have trimeric structures that assemble into hexameric and higher order molecular forms. C1qTNF3, also known as CORS26, is predominantly expressed in cartilage and is induced in mature adipocytes. C1qTNF10, also known as C1qL2, is a protein that shows 28% - 30% sequence identity with C1q subunits A, B, and C. It is predicted to contain one collagen-like and C1q domain. Mouse and human C1qTNF10 share over 90% amino acid sequence identity.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
>50 ug/mL as determined by microplate BCA method