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Recombinant Human C3 protein, His-tagged

Cat.No. : C3-7631H
Product Overview : Recombinant Human C3 aa. (Glu1322~Cys1661) fused with N-terminal His tag was produced in E. coli cells.
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  • Gene Information
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Description : Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 46kDa as determined by SDS-PAGE reducing conditions.
Protein length : Glu1322~Cys1661
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >92%
Characteristic : The isoelectric point is 4.9.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Reconstitution : Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : C3 complement C3 [ Homo sapiens (human) ]
Official Symbol : C3
Synonyms : ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; acylation-stimulating protein cleavage product; complement component 3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; prepro-C3; complement C3
Gene ID : 718
mRNA Refseq : NM_000064.3
Protein Refseq : NP_000055.2
UniProt ID : P01024

Products Types

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◆ Native Protein
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Have proteins been found to interact with C3 proteins? 10/26/2019

Yes, a variety of proteins that interact with C3 proteins, including complement receptors, regulatory proteins, inflammatory factors, etc., have been discovered, and they are jointly involved in the regulation and functional execution of C3 proteins.

What is the relationship between C3 protein and infectious diseases? 09/28/2019

C3 protein plays an important role in infectious diseases. It can play an anti-infection role by activating the complement system, directly killing pathogens or enhancing the ability of immune cells to destroy pathogens.

How is the research progress of C3 protein at present? 09/23/2019

Research on C3 protein is going on. At present, it mainly focuses on the analysis of its function and regulatory mechanism, the identification of gene mutations associated with diseases, and the interaction with other complement proteins and immune cells, which provides a theoretical basis for the further development of diagnosis and treatment methods of related diseases.

What is the use of C3 protein in laboratory diagnostics? 05/24/2019

C3 protein can be used as an aid in the diagnosis of certain immune-related diseases. Measuring the level of C3 protein in serum can reflect the activity of the immune system and the degree of inflammation.

What are the effects of post-translational modifications of C3 protein on its function? 04/15/2019

Post-translational modifications of the C3 protein can affect its function. Phosphorylation, cleavage, methylation and other modifications can regulate the activity, stability and interaction ability of C3 proteins.

Have any drug treatments associated with C3 protein been identified? 02/22/2019

Yes, some C3 protein-related drug treatments are under development, including drugs that regulate the degree of C3 protein activation, drugs that inhibit the overactivation of C3 protein, etc.

Customer Reviews (3)

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Reviews
02/23/2022

    Expression efficiency is high. In our experiments, we found that the target protein can be synthesized quickly and effectively.

    01/26/2022

      Activity was not significantly different from the native protein, as expected.

      03/23/2021

        High purity, no obvious impurities.

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