Recombinant Human CBX1(1-185 aa)(3XMBT DOMAIN, D355N) fused with GST tag at N-terminal was expressed in E. coli.
L3MBTL1 3xMBT contains the three MBT repeat domains present in the protein. L3MBTL1 is a member of the Polycomb group of proteins and binds to broad range of proteins that exhibit mono- and dimethylation, including histone H1, histone H4, p53 and Rb. The truncated version of L3MBTL1 present in the 3xMBT domain protein serves as a useful tool for the detection of mono- and dimethylation of a wide variety of proteins.
In 50 mM Tris-HCl pH 7.5, 300 mM NaCl, 1.5 mM DTT, 20% glycerol.
L3MBTL1 3xMBT Domain, D355N, Recombinant Human is useful as a non-binding control protein when studying wild type L3MBTL1.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.