Recombinant Human CCS Protein, with Cu (I)

• Cat.No.: CCS-483H
• Product Overview: Recombinant full length Human Cu (I)-CCS (aa 1-274)(Copper chaperone for superoxide dismutase) with a GSFT sequence at N-terminal was expressed in E. coli. Binding of 2 Cu (I) to the protein was confirmed by ICP.

Specification

• Description: Copper chaperone for superoxide dismutase specifically delivers Cu to copper/zinc superoxide dismutase and may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
• Source: E. coli
• Species: Human
• Form: In 20 mM Phosphate buffer pH 7.0.
• Molecular Mass: 29.4 kDa
• Protein length: aa 1-274
• AA Sequence: GMSF-MASDSGNQGT LCTLEFAVQM TCQSCVDAVR KSLQGVAGVQ DVEVHLEDQM VLVHTTLPSQ EVQALLEGTG RQAVLKMGS GQLQNLGAAV AILGGPGTVQ GVVRFLQLTP ERCLIEGTID GLEPGLHGLH VHQYGDLTMM CNSCGNHFNP DGASHGGPQD SDRHRGDLGN VRADADGRAI FRMEDEQLKV WDVIGRSLII DEGEDDLGRG GHPLSKITGN SGERLACGII ARSAGLFQNP KQICSCDGLT IWEERGRPIA GKGRKESAQP PAHL
• Purity: >95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight > 25 kDa and < 35 kDa.
• Notes: To avoid oxidation and leaking of the Cu(I) bound to the protein, keep and use the sample under inert atomosphere.
• Storage: Store at - 20 centigrade. The protein is stable at 4 centigrade for at least 2 weeks and at 25 centigrade for at least several hours. After initial deforst, aliquot product into individual tubes under inert atomosphere and refreeze at - 20 centigrade. Avoid repeated freeze/defrost cycles. Avoid exposition to air.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: CCS copper chaperone for superoxide dismutase [ Homo sapiens ]
• Official Symbol: CCS
• Synonyms: CCS; copper chaperone for superoxide dismutase; superoxide dismutase copper chaperone; MGC138260
• Gene ID: 9973
• mRNA Refseq: NM_005125
• Protein Refseq: NP_005116
• MIM: 603864
• UniProt ID: O14618
• Chromosome Location: 11
• Pathway: Amyotrophic lateral sclerosis (ALS), organism-specific biosystem; Amyotrophic lateral sclerosis (ALS), conserved biosystem
• Function: copper ion binding; copper ion transmembrane transporter activity; metal ion binding; protein binding; NOT superoxide dismutase activity; superoxide dismutase copper chaperone activity; zinc ion binding