Recombinant human CD177 (NTN4) gene (22 - 408 aa) fused with T7/His tag at N-terminal was expressed in E. coli.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFELLLCQFGTVQHVWKVSDLPRQWTPKNTSCDSGLGCQDTLMLIESG PQVSLVLSKGCTEAKDQEPRVTEHRMGPGLSLISYTFVCRQEDFCNNLVNSLPLWAPQPPADPGSLRCPVCLSME GCLEGTTEEICPKGTTHCYDGLLRLRGGGIFSNLRVQGCMPQPGCNLLNGTQEIGPVGMTENCNRKDFLTCHRGT TIMTHGNLAQEPTDWTTSNTEMCEVGQVCQETLLLLDVGLTSTLVGTKGCSTVGAQNSQKTTIHSAPPGVLVASY THFCSSDLCNSASSSSVLLNSLPPQAAPVPGDRQCPTCVQPLGTCSSGSPRMTCPRGATHCYDGYIHLSGGGLST KMSIQGCVAQPSSFLLNHTRQIGIFSAREKRDVQPPASQHEGG
1. Protein can be used as coating matrix protein for study human neutrophil-endothelia cell interaction study in vitro.2. As highly purified recombinant antigen, it may be used as culture matrix protein for differentiation of human endothelial cell in vitro.
Keep at -20centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.