Active Recombinant Human CD28 protein, Biotin-labelled

• Cat.No.: CD28-32H
• Product Overview: Human CD28-muIg fusion protein was Protein A purified from (low FBS containing) tissue culture supernatant of CHO transfectants, and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate by diafiltration. A soluble fusion protein consisting of the extracellular (134aa) domain of human CD28 fused to murine IgG2a Fc (233aa).

Specification

• Description: Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60 kd and 80 kd, respectively.
• Source: CHO
• Species: Human
• Bio-activity: CD28-muIg/Biotin activity was assessed in EIA utilizing GAM-captured Recombinant CD80-muIg, and Streptavidin/HRP as a detector. In this format, the fusion protein was detectable at 100 ng/ml.
• Notes: Research use only. Not for use in Diagnostic procedures.
• Stability: Product should retain activity for at least 12 months after shipping date when stored as recommended.
• Storage: Store at 2 - 5 centigrade. Freeze/Thawing is not recommended.
• Concentration: 0.5 mg/mL
• Storage Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).

Gene Information

• Gene Name: CD28
• Official Symbol: CD28 CD28 molecule [ Homo sapiens (human) ]
• Synonyms: CD28; CD28 molecule; Tp44; T-cell-specific surface glycoprotein CD28; CD28 antigen
• Gene ID: 940
• mRNA Refseq: NM_006139
• Protein Refseq: NP_006130
• MIM: 186760
• UniProt ID: P10747