Active Recombinant Human CD3E protein, His-tagged

• Cat.No.: CD3E-213H
• Product Overview: Recombinant Human CD3E protein(Asp 23 - Asp 126), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human CD3 epsilon, His Tag at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human CD3 (SP34-2) with a linear range of 0.2-3 ng/mL.Immobilized Mouse Anti Human CD3(SP-34) at 2 μg/mL (100 μL/well) can bind Human CD3 epsilon, His Tag with a linear range of 0.2-2 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.9 kDa. As a result of PTMs, the protein migrates as 20-21 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
• Proteinlength: Asp23-Asp126
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at: -20°C to -70°C for 24 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies (2019)

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766