Recombinant human CD47, which carry single Ig-like V type domain cDNA (19 – 127 aa, derived from BC012884) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
1. May be used for in vitro non-glycosylated CD47 longest extracellular domain protein mediated "don"t eat me" signal regulation study for cancer cell imgration, T cell activation et al. with this protein as either coating matrix protein or soluble factor.2. May be used as CD47 protein-protein interaction assay.3. As enzymatic substrate for various proteases.4. As potential cancer diagnostic biomarker protein.5. As antigen for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.