Recombinant human CD69, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CD69, also known as early activation antigen CD69, is a type 2 transmembrane glycoprotein in the C-type lectin family. Also, CD69 is a very early activation Ag of T lymphocytes. It is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. This protein plays a role in the pathogenesis of allergen induced eosinophilic airway inflammation and responsiveness.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
17.0kDa (147aa)18-28KDa (SDS–PAGE under reducing conditions)
ADPSVGQYNC PGQYTFSMPS DSHVSSCSED WVGYQRKCYF ISTVKRSWTS AQNACSEHGA TLAVIDSEKD MNFLKRYAGR EEHWVGLKKE PGHPWKWSNG KEFNNWFNVT GSDKCVFLKN TEVSSMECEK NLYWICNKPY KHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>95% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)