Recombinant Human CD99 protein, His-tagged
Recombinant Human CD99(Asp 23 - Asp 122) fused with His tag at N-terminal was expressed in HEK293.
CD99 antigen is also known as MIC2, single-chain type-1 glycoprotein, HBA71, MIC2X, MIC2Y, MSK5X, 12E7, which belongs to the CD99 family. CD99 / MIC2 is expressed on all leukocytes but highest on thymocytes. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. CD99 / MIC2 plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. CD99 acts at the same site as, but independently of PECAM1. There is also experimental evidence that it binds to cyclophilin A.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 11 kDa. The reducing (R) protein migrates as 25 kDa in SDS-PAGE due to glycosylation.
Less than 1.0 EU per μg of the Human CD99, His Tag by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4centigrade); After reconstitution under sterile conditions for 3 months (-70centigrade).