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Home > Products > Recombinant Proteins > Recombinant Human CDC42(Q61L) protein, His-tagged

Recombinant Human CDC42(Q61L) protein, His-tagged

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Cat.No.:
CDC42-2637H
Product Overview:
Recombinant Human CDC42 fused with a histidine tag in the amino-terminus was expressed in E. coli.
Description:
The constitutively active form of human Cdc42 protein contains a glutamine to leucine substitution at residue 61. The common name for this mutant is Cdc42(Q61L) or L61Cdc42. The leucine substitution prevents endogenous and GAP-stimulated GTPase activity of Cdc42, hence the protein is always in the active, GTP-bound, state. A similar mutation in Ras (Ras(Q61L)) behaves as an oncogenic mutation.
Source:
E. coli
Species:
Human
Tag :
His
Form:
2 mM Tris pH 7.6, 0.5 mM MgCl2, 0.5% sucrose and 0.1% dextran.
Molecular Mass:
~25 kDa
Purity:
>70% pure
Applications:
Cdc42 effector and GAP identification and binding studies; Activation of Cdc42 effectors in vitro and in vivo; Stimulation of Cdc42 phenotype by microinjection into cells


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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