Recombinant human CDCP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CDCP1, also known as CUB domain-containing protein 1 isoform 1, is a transmembrane protein containing three extracellular CUB domains. This protein is involved in cell adhesion and cell matrix association. Also, CDCP1 may play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation and may be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. The extracellular region of human CDCP1 shares amino acid identity sequence with that of the mouse protein.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
72.8kDa (646aa)70-100KDa (SDS–PAGE under reducing conditions)
FEIALPRESN ITVLIKLGTP TLLAKPCYIV ISKRHITMLS IKSGERIVFT FSCQSPENHF VIEIQKNIDC MSGPCPFGEV QLQPSTSLLP TLNRTFIWDV KAHKSIGLEL QFSIPRLRQI GPGESCPDGV THSISGRIDA TVVRIGTFCS NGTVSRIKMQ EGVKMALHLP WFHPRNVSGF SIANRSSIKR LCIIESVFEG EGSATLMSAN YPEGFPEDEL MTWQFVVPAH LRASVSFLNF NLSNCERKEE RVEYYIPGST TNPEVFKLED KQPGNMAGNF NLSLQGCDQD AQSPGILRLQ FQVLVQHPQN ESNKIYVVDL SNERAMSLTI EPRPVKQSRK FVPGCFVCLE SRTCSSNLTL TSGSKHKISF LCDDLTRLWM NVEKTISCTD HRYCQRKSYS LQVPSDILHL PVELHDFSWK LLVPKDRLSL VLVPAQKLQQ HTHEKPCNTS FSYLVASAIP SQDLYFGSFC PGGSIKQIQV KQNISVTLRT FAPSFQQEAS RQGLTVSFIP YFKEEGVFTV TPDTKSKVYL RTPNWDRGLP SLTSVSWNIS VPRDQVACLT FFKERSGVVC QTGRAFMIIQ EQRTRAEEIF SLDEDVLPKP SFHHHSFWVN ISNCSPTSGK QLDLLFSVTL TPRTVDLTLE HHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)