Recombinanat human CDH1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CDH1, also known as cadherin-1, is a member of cell surface glycoproteins that meditate cell adhesion. Human CDH1 shares amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that meditates calcium-dependent epithelial cell adhesion. This protein preferentially interacts with themselves in a homophilic manner in connecting cells. It may thus contribute to the sorting of heterogeneous cell types. It is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. It is a ligand for integrin alpha-E/beta-7.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
76.6kDa (694aa)70KDa (SDS–PAGE under reducing conditions)
EPEPCHPGFD AESYTFTVPR RHLERGRVLG RVNFEDCTGR QRTAYFSLDT RFKVGTDGVI TVKRPLRFHN PQIHFLVYAW DSTYRKFSTK VTLNTVGHHH RPPPHQASVS GIQAELLTFP NSSPGLRRQK RDWVIPPISC PENEKGPFPK NLVQIKSNKD KEGKVFYSIT GQGADTPPVG VFIIERETGW LKVTEPLDRE RIATYTLFSH AVSSNGNAVE DPMEILITVT DQNDNKPEFT QEVFKGSVME GALPGTSVME VTATDADDDV NTYNAAIAYT ILSQDPELPD KNMFTINRNT GVISVVTTGL DRESFPTYTL VVQAADLQGE GLSTTATAVI TVTDTNDNPP IFNPTTYKGQ VPENEANVVI TTLKVTDADA PNTPAWEAVY TILNDDGGQF VVTTNPVNND GILKTAKGLD FEAKQQYILH VAVTNVVPFE VSLTTSTATV TVDVLDVNEA PIFVPPEKRV EVSEDFGVGQ EITSYTAQEP DTFMEQKITY RIWRDTANWL EINPDTGAIS TRAELDREDF EHVKNSTYTA LIIATDNGSP VATGTGTLLL ILSDVNDNAP IPEPRTIFFC ERNPKPQVIN IIDADLPPNT SPFTAELTHG ASANWTIQYN DPTQESIILK PKMALEVGDY KINLKLMDNQ NKDQVTTLEV SVCDCEGAAG VCRKAQPVEA GLQIPALEHH HHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS – PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)