Recombinant Human CDH1(Gln 23 - Pro 621) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Has a calculated MW of 92.4 kDa. As a result of different glycosylation, DTT-reduced Protein migrates as 110-125 kDa Cadherin, a 48kDa Propeptide and non-reduced protein migrates as 200-250 kDa in SDS-PAGE .
Gln 23 - Pro 621
Less than 1.0 EU per μg of the Human E-Cadherin, Fc Tag by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).