Recombinant Human CDH3(Asp108 - Gly654) fused with His/Fc tag at C-terminal was expressed in NS0.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by the ability of the immobilized protein to support the adhesion of A431 human epithelial carcinoma cells. When 1 x 10^5 cells/well are added to rhP-CAD/Fc Chimera coated plates (10 μg/mL with 100 μL/well), approximately 30-60% will adhere after 1.5 hours at 37° C.Optimal concentration depends on cell type as well as the application or research objectives.
Predicted Molecular Mass: 87.4 kDa;SDS-PAGE: 120-130 kDa, under reducing conditions.
<0.01 EU per 1 μg of the protein by the LAL method.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.