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Active Recombinant Human CDK2/CCNE1 protein, GST-tagged

Cat.No. : CDK2-48H
Product Overview : Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
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Description : CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
Source : Insect cells
Species : Human
Tag : GST
Form : 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Bio-activity : 404 nmol/min/mg
Molecular Mass : CDK2 ~58kDa and CyclinE1 ~73kDa
Purity : >90%
Applications : Kinase Assay, Western Blot
Stability : 1 year at -70 centigrade from date of shipment
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1μg/μl
Gene Name : CDK2 cyclin-dependent kinase 2 [ Homo sapiens ]
Official Symbol : CDK2
Synonyms : CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2);
Gene ID : 1017
mRNA Refseq : NM_001798
Protein Refseq : NP_001789
MIM : 116953
UniProt ID : P24941
Chromosome Location : 12q13
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of ATR in response to replication stress, organism-specific biosystem
Function : ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase activity; contributes_to histone kinase activity; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the primary role of CDK2 in cell cycle regulation? 07/10/2021

CDK2 is crucial for driving cells through the G1/S and S phases of the cell cycle.

What role does CDK2 play in cellular response to DNA damage? 05/04/2020

CDK2 helps in cell cycle arrest to allow DNA repair, preventing mutation accumulation.

What are the key regulatory mechanisms controlling CDK2 activity? 03/08/2020

CDK2 activity is controlled by cyclins, CDK inhibitors, and phosphorylation status.

How is dysregulation of CDK2 implicated in cancer development? 01/23/2020

CDK2 dysregulation can lead to uncontrolled cell proliferation, contributing to cancer.

Are there existing or potential therapeutic strategies targeting CDK2? 01/08/2020

Targeting CDK2 with specific inhibitors is explored as a potential cancer therapy strategy.

How is CDK2 activity linked to cell cycle checkpoints? 04/06/2019

It is integral to cell cycle checkpoints ensuring DNA integrity before replication.

How does CDK2 contribute to DNA replication? 02/13/2018

It phosphorylates proteins necessary for DNA replication initiation.

Customer Reviews (3)

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Reviews
04/14/2022

    Product met expectations, happy.

    03/22/2022

      High quality, reliable results.

      05/31/2021

        Outstanding performance, highly recommend.

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