Active Recombinant Human CDK2/CCNE1 protein, GST-tagged
Cat.No. : | CDK2-48H |
Product Overview : | Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. |
- Specification
- Gene Information
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Description : | CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition. |
Source : | Insect cells |
Species : | Human |
Tag : | GST |
Form : | 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol. |
Bio-activity : | 404 nmol/min/mg |
Molecular Mass : | CDK2 ~58kDa and CyclinE1 ~73kDa |
Purity : | >90% |
Applications : | Kinase Assay, Western Blot |
Stability : | 1 year at -70 centigrade from date of shipment |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
Concentration : | 0.1μg/μl |
Gene Name : | CDK2 cyclin-dependent kinase 2 [ Homo sapiens ] |
Official Symbol : | CDK2 |
Synonyms : | CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2); |
Gene ID : | 1017 |
mRNA Refseq : | NM_001798 |
Protein Refseq : | NP_001789 |
MIM : | 116953 |
UniProt ID : | P24941 |
Chromosome Location : | 12q13 |
Pathway : | APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Activation of ATR in response to replication stress, organism-specific biosystem |
Function : | ATP binding; cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase activity; contributes_to histone kinase activity; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; |
Products Types
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Cdk2-864M | Recombinant Mouse Cdk2 Protein, MYC/DDK-tagged | +Inquiry |
CDK2-3142H | Recombinant Human CDK2 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
CDK2-708HCL | Recombinant Human CDK2 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionCDK2 is crucial for driving cells through the G1/S and S phases of the cell cycle.
CDK2 helps in cell cycle arrest to allow DNA repair, preventing mutation accumulation.
CDK2 activity is controlled by cyclins, CDK inhibitors, and phosphorylation status.
CDK2 dysregulation can lead to uncontrolled cell proliferation, contributing to cancer.
Targeting CDK2 with specific inhibitors is explored as a potential cancer therapy strategy.
It is integral to cell cycle checkpoints ensuring DNA integrity before replication.
It phosphorylates proteins necessary for DNA replication initiation.
Customer Reviews (3)
Write a reviewProduct met expectations, happy.
High quality, reliable results.
Outstanding performance, highly recommend.
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