Recombinant full-length human CDK2 and CCNE2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.
CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed. CDK2 is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. CDK2 associates with and is regulated by the regulatory subunits of the complex including Cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). CDK2 phosphorylates multiple cellular substrates including SMAD3 and FOXO1. Phosphorylation of FOXO1 leads to its inhibition.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol
The specific activity of CDK2/CyclinE2 was determined to be 60nmol/min/mg as per activity protocol.
CDK2 ~58 kDa and CCNE2 ~72 kDa
The purity of CDK2/CCNE2 was determined to be >90% by densitometry.
Kinase Assay; WB
1 year at -70 centigrade
Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.