Recombinant Human CDK4(Met1-Glu303) fused with His/T7 tag at C-terminal was expressed in E. coli.
Protein in [20mM PB,150mM NaCl,pH7.4] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
Less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test.
Greater than 95% as determined by reducing SDS-PAGE.
Lyophilized form is stable at room temperature for 3 weeks. Keep at -20 centigrade for long term storage. After reconstitution, store at 4 centigrade for 2-7 days or at below -20 centigrade for up to 3 months.
Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.