Recombinant Human CDK4 protein, His/T7-tagged

• Cat.No.: CDK4-938H
• Product Overview: Recombinant Human CDK4(Met1-Glu303) fused with His/T7 tag at N-terminal was expressed in E. coli.

Specification

• Description: Cyclin-Dependent Kinase 4 (CDK4) is a member of the CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK4 is a component of Cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G1/S transition. These complexes are major integrators of various mitogenenic and antimitogenic signals. It is shown that CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb). Defects in CDK4 are a cause of susceptibility to cutaneous Malignant Melanoma Type 3.
• Source: E. coli
• Species: Human
• Tag: His/T7
• Form: Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
• Protein length: Met1-Glu303
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMASMT GGQQMGRGSMATSRYEPVAEIGVGA YGTVYKARDPHSGHF VALKSVRVPNGGGGGGGLPISTVRE VALLRRLEAFEHPNVVRLMDVCATS RTDREIKVTLVFEHV DQDLRTYLDKAPPPGLPAETIKDLM RQFLRGLDFLHANCIVHRDLKPENI LVTSGGTVKLADFGL ARIYSYQMALTPVVVTLWYRAPEVL LQSTYATPVDMWSVGCIFAEMFRRK PLFCGNSEADQLGKI FDLIGLPPEDDWPRDVSLPRGAFPP RGPRPVQSVVPEMEESGAQLLLEML TFNPHKRISAFRALQ HSYLHKDEGNPE
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
  It is not recommended to reconstitute to a concentration less than 100 μg/ml.
  Dissolve the lyophilized protein in ddH2O.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: CDK4 cyclin-dependent kinase 4 [ Homo sapiens ]
• Official Symbol: CDK4
• Synonyms: CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458
• Gene ID: 1019
• mRNA Refseq: NM_000075
• Protein Refseq: NP_000066
• MIM: 123829
• UniProt ID: P11802
• Chromosome Location: 12q13
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase activity