Active Recombinant Human CEACAM1 protein, His-tagged

• Cat.No.: CEACAM1-152H
• Product Overview: Recombinant Human CEACAM1(Gln35-Gly428) fused with His tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln35
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit IL-2 secretion by HuT 78 human cutaneous T cell lymphoma cells Chen, C.J. and J.E. Shively (2004) J. Immunol. 172:3544. rhCEACAM-1 at concentrations higher than 1 µg/mL reduces anti-CD3 (immobilized at 10 µg/mL) induced IL-2 production by 60-70%.The ED50 for rhCEACAM-1 inhibition of anti-CD3 induced IL-2 production is typically 0.05-0.2 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 44.6 kDa;SDS-PAGE: 100-130 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: CEACAM1 carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) [ Homo sapiens ]
• Official Symbol: CEACAM1
• Synonyms: CEACAM1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); BGP; carcinoembryonic antigen-related cell adhesion molecule 1; BGP1; CD66a; antigen CD66; CD66a antigen; BGPI
• Gene ID: 634
• mRNA Refseq: NM_001024912
• Protein Refseq: NP_001020083
• MIM: 109770
• UniProt ID: P13688
• Chromosome Location: 19q13.2
• Pathway: EGFR1 Signaling Pathway, organism-specific biosystem
• Function: molecular_function; protein binding