Recombinant human CECR1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CECR1, also known as adenosine deaminase CECR1 isoform, is a member of a family of adenosine deaminase-related growth factors. Adenosine deaminase is one of the key enzymes of purine nucleotide metabolism. CECR1 is a secreted protein that is expressed in many tissues, with the highest expression in lymphoblasts, heart, lung, and placenta.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
56.9kDa (490aa)50-70KDa (SDS–PAGE under reducing conditions.)
IDETRAHLLL KEKMMRLGGR LVLNTKEELA NERLMTLKIA EMKEAMRTLI FPPSMHFFQA KHLIERSQVF NILRMMPKGA ALHLHDIGIV TMDWLVRNVTYRPHCHICFT PRGIMQFRFA HPTPRPSEKC SKWILLEDYR KRVQNVTEFD DSLLRNFTLV TQHPEVIYTN QNVVWSKFET IFFTISGLIH YAPVFRDYVFRSMQEFYEDN VLYMEIRARL LPVYELSGEH HDEEWSVKTY QEVAQKFVET HPEFIGIKII YSDHRSKDVA VIAESIRMAM GLRIKFPTVV AGFDLVGHEDTGHSLHDYKE ALMIPAKDGV KLPYFFHAGE TDWQGTSIDR NILDALMLNT TRIGHGFALS KHPAVRTYSW KKDIPIEVCP ISNQVLKLVS DLRNHPVATLMATGHPMVIS SDDPAMFGAK GLSYDFYEVF MGIGGMKADL RTLKQLAMNS IKYSTLLESE KNTFMEIWKK RWDKFIADVA TKLEHHHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)