Recombinant Human CHCHD4, was expressed in E. coli.
Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0.
WB, ELISA, IP, antibody production, protein array
Store at -80°C and avoid repeated freeze-thaw cycles