Recombinant human CNTF protein was expressed in E.coli and purified by using conventional chromatography techniques.
CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding.
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is less than or equal to 500 ng/ml.
22.9 kDa (200aa), confirmed by MALDI-TOF
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARLLEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
1 mg/ml (determined by Bradford assay)