Recombinant human Collagen VI extracellular domain cDNA (27 - 227 aa) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGETELSVAQCTQRPVDIVFLLDGSERLGEQNFHKARRFVEQVARRLTLA RRDDDPLNARVALLQFGGPGEQQVAFPLSHNLTAIHEALETTQYLNSFSHVGAGVVHAINAIVRSPRGRARRHAE LSFVFLTDGVTGNDSLHESAHSMRKQNVVPTVLALGSDVDMDVLTTLSLGDRAAVFHEKDYDSLAQPGFFDRFIR WIC
1. May be used for in vitro various collagen VI / integrin binding regulation for cell lineage specific differentiations study coating with this protein.2. May be used for protein-protein interaction assay.3. As antigen for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.