Recombinant Human CSF1R(Ile 20 - Glu 512) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.5. Normally Trehalose is added as protectant before lyophilization.
Measured by its ability to inhibit the MCSF-induced proliferation of NFS-60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is typically 4-12 pg/mL in the presence of 1 ng/mL of rhMCSF.
Has a calculated MW of 80.7 kDa. The reducing (R) protein migrates as 95-150 kDa in SDS-PAGE due to glycosylation.
Ile 20 - Glu 512
Less than 0.1 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).