Recombinant human CTLA4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
CTLA4, also known as cytotoxic T-lymphocyte-associated protein 4, is a co-inhibitory molecule expressed on T cells that mediates the inhibition of T-cell function. This protein is a crucial immune regulator that mediates both negative costimulation signals to T cells, and regulatory T (Treg)-cell extrinsic control of effector responses. It has been implicated in several autoimmune diseases, such as systemic lupus erythematosus.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 20% glycerol.
14.6kDa (135aa)18-28KDa (SDS–PAGE under reducing conditions.)
ADLKAMHVAQ PAVVLASSRG IASFVCEYAS PGKATEVRVT VLRQADSQVT EVCAATYMMG NELTFLDDSI CTGTSSGNQV NLTIQGLRAM DTGLYICKVE LMYPPPYYLG IGNGTQIYVI DPEPCPDSDH HHHHH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
>90% by SDS - PAGE
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
0.25mg/ml (determined by Absorbance at 280nm)