Recombinant Human CTSL(18-333 aa) fused with His tag at N-terminal was expressed in E. coli.
CTSL, also known as cathepsin L1 isoform 1, is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene.
Liquid. In 20mM Tris-HCl(pH8.0) containing 10% glycerol
38.3kDa (339 aa) confirmed by MALDI-TOF
MGSSHHHHHH SSGLVPRGSH MGSTLTFDHS LEAQWTKWKA MHNRLYGMNE EGWRRAVWEK NMKMIELHNQ EYREGKHSFT MAMNAFGDMT
SEEFRQVMNG FQNRKPRKGK VFQEPLFYEA PRSVDWREKG YVTPVKNQGQ CGSCWAFSAT GALEGQMFRK TGRLISLSEQ NLVDCSGPQG
NEGCNGGLMD YAFQYVQDNG GLDSEESYPY EATEESCKYN PKYSVANDTG FVDIPKQEKA LMKAVATVGP ISVAIDAGHE SFLFYKEGIY
FEPDCSSEDM DHGVLVVGYG FESTESDNNK YWLVKNSWGE EWGMGGYVKM AKDRRNHCGI ASAASYPTV
< 1.0 EU per 1 microgram of protein (determined by LAL method)
> 85% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
1 mg/ml (determined by Bradford assay)