Recombinant Human CYP3A4/NADPH reductase protein

• Cat.No.: CYP3A4-33H
• Product Overview: This is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP 3A4 and recombinant human P450 NADPH oxidoreductase co-expressed in the same preparation.

Specification

• Source: Pichia pastoris
• Species: Human
• Form: Lyophilized powder
• Molecular Mass: 55 Kda
• Characteristic: - Co-expresses human CYP3A4 and human P450 oxidoreductase (hCPR) allowing easy reconstitution of the endogenous system- Useful for all the applications as a baculosome system but more cost-effective- Easy to handle dry powder- Very stable in dry form and active in multiple buffers and solvents during long incubations- Addition of NADP+ & Glucose-6-Phosphate are not essential (but will boost T3activity)- After the reaction, the protein can be pelleted at relatively low speeds- Clean HPLC profiles for easy metabolite ID and purification- Highly controlled production process for lot-to-lot reproducibility
• Notes: Centrifuge the vial prior to opening
• Storage: Stable for 1 year as supplied. Store dry powder at -20 centigrade. Reconstitute in 100 mM Potassium Phosphate buffer, pH 7.7. Not recommended to be stored as solution. If storing as solution, aliquot and store at -80 centigrade. Avoid repeated freeze/thaw cycles and use aliquots within one month (the human CYP3A4 will lose approximately 10% activity per week when stored at -80 centigrade). Thaw aliquots rapidly at 37 centigrade and place on ice until use (thawed aliquots should be used within 4 hours).
• Reconstitution: 100 mM Potassium Phosphate buffer, pH 7.7

Gene Information

• Gene Name: CYP3A4 cytochrome P450, family 3, subfamily A, polypeptide 4 [ Homo sapiens ]
• Official Symbol: CYP3A4
• Synonyms: CYP3A4; cytochrome P450, family 3, subfamily A, polypeptide 4; CYP3A3, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1; MGC126680
• Gene ID: 1576
• mRNA Refseq: NM_001202855
• Protein Refseq: NP_001189784
• MIM: 124010
• UniProt ID: P08684
• Chromosome Location: 7q21.1
• Pathway: Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Biological oxidations, organism-specific biosystem; Codeine and morphine metabolism, organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Drug metabolism - cytochrome P450, organism-specific biosystem; Drug metabolism - cytochrome P450, conserved biosystem
• Function: albendazole monooxygenase activity; caffeine oxidase activity; electron carrier activity; enzyme binding; heme binding; iron ion binding; metal ion binding; monooxygenase activity; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding; quinine 3-monooxygenase activity; steroid binding; steroid hydroxylase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; testosterone 6-beta-hydroxylase activity; vitamin D 24-hydroxylase activity; vitamin D3 25-hydroxylase activity