Recombinant human DDIT3 cDNA (169 aa, isoform 2) fused with T7-His-TEV cleavage site Tag (29aa) at N-terminal was expressed in E. coli.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFAAESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEE ESKIFTTLDPASLAWLTEEEPEPAEVTSTSQSPHSPDSSQSSLAQEEEEEDQGRTRKRKQSGHSPARAGKQRMKE KEQENERKVAQLAEENERLKQEIERLTREVEATRRALIDRMVNLHQA
1. May be used for in vitro stress mediated DNA damage pathway regulation study with intracellular delivery of recombinant DDIT3 protein technology, such as PULSinTM protein transfaction system.2. May be used as specific substate protein for kinase or ubiquitin ligase assay development.3. May be used as antigen for specific antibody production.
Keep at -80°C for long term storage. Product is stable at 4 °C for at least 30 days.