Recombinant Human DOT1L(amino acids 1 to 472) fused with GST tag at N-terminal was expressed in E. coli.
100 mM Tris pH 8.0, 5 mM glutathione and 25% glycerol.
Recombinant DOT1L Catalytic Domain, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 3-5 µg DOT1L per reaction with HeLa or recombinant nucleosomes as a substrate is recommended.
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.