Recombinant Human DOT1L(1-472 aa)(Catalytic Domain) fused with GST tag at N-terminal was expressed in E. coli.
DOT1 is a histone methyltransferase, catalyzing the methylation of histone H3 at lysine 79 on nucleosomal substrates.
100 mM Tris pH 8.0, 5 mM glutathione and 25% glycerol.
Recombinant DOT1L Catalytic Domain, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 3-5 µg DOT1L per reaction with HeLa or recombinant nucleosomes as a substrate is recommended.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.