Human DRD1 derived in Human HEK293T cell line.
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
Human HEK293T cell line
1 vial of 100 ug gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 ?g empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
The lysate is shipped with dry ice. Upon receiving, store the sample at -20 centigrade. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.