Recombinant Human EBF1(4-350aa) fused with GST tag at N-terminal was expressed in E. coli.
EBF1 played an important role in many functions.
1M PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, pH8.0 ), 100 mM GSH and 1% Triton X-100, 15% glycerol.
IQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQ GQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRV LLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAV SDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSE LITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEP
Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months. Avoid freeze thaw cycles.Storage of Reconstituted Protein:Short-term storage: Store at 2-8 centigrade for two weeks.Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buﬀer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Reconsititute at 0.25 µg/μl in 200 μl sterile water for short-term storage.Reconsitution with 200 μl 50% glycerol solution is recommended for longer term storage.If a different concentration is needed for your purposes please adjust the reconsitution volume as required (please note: the ion concentration of the ﬁnal solution will vary according to the volume used).Note: Centrifuge vial before opening. When reconsituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
The product is shipped with ice packs. Upon receipt, store it immediately at -20 centigrade to -80 centigrade.