Recombinant Human EDN1 fused with His tag at N-terminal was expressed in E. coli.
Endothelin 1, also known as preproendothelin-1 (PPET1), is a protein that in humans is encoded by the EDN1 gene. The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 is one of three isoforms of human endothelin (ET-1).Preproendothelin is precursor of the peptide ET-1. Endothelial cells convert preproendothelin to proendothelin and subsequently to mature endothelin, which the cells release.
Lyophilized from 50mM Tris-HCL，0.5mM EDTA，50mM NaCl, pH 7.4
> 90% as determined by SDS-PAGE
Western blotting, ELISA
Store it under sterile conditions at -20 centigrade to -80 centigrade upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Reconstitute in ddH2O