Recombinant Human EGFR, cytoplasmic domain [669-1210(end) amino acids and T790M/L858R of accession numberNP_005219.2] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
>80% as determined by SDS-PAGE and CBB staining
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.