Recombinant human EGFR (d746-750 T790M C797S L858R) (668-end) fused with an N-terminal GST tag was expressed in insect cells.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
The specific activity was determined to be 150 nmol/min/mg.
>70% by densitometry
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.