Recombinant Human EGFR (d746-750) (668-end) fused with GST tag at N-terminal was expressed in Sf9 insect cells.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
The specific activity was determined to be 54 nmol/min/mg.
>70% by densitometry
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.