Recombinant Human EHMT2(913-1210 aa) fused with His tag at N-terminal was expressed in E. coli.
G9a is a SET-domain containing Histone methyltransferase, catalyzing the mono- and di-methylation of Histone H3 at lysine 9.
100mM Tris pH 8.0, 10 mM glutathione and 25% glycerol.
G9a, Recombinant Human, His-tagged is useful for Histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 1-3 µg G9a per reaction with nucleosomes or recombinant Histone H3 as a substrate is recommended. Dilute as needed before using.
Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.