Recombinant human ENTPD5 (O75356) (Met 1-His 428) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 5%~8% trehalose and mannitol.
Measured by its ability to hydrolyze the 5"phosphate groups from the substrate guanosine5"diphosphate (GDP).The specific activity is >7,000 pmoles/min/μg.
The secreted recombinant human ENTPD5 consists of 418 amino acids and predicts a molecular mass of 47 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.