Recombinant human EPHA1 (EAL23789.1) (Met1-Glu547) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5% ~ 8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA.Immobilized human EPHA1-His at 10 μg/ml (100 μl /well) can bind human EFNA1-Fch, The EC50 of human EFNA1-Fch is 30-70 ng/ml.
The recombinant human EPHA1 consists 533 amino acids and predicts a molecular mass of 58 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.