Active Recombinant Human EPHA1 Protein, His-tagged

• Cat.No.: EPHA1-727H
• Product Overview: Recombinant human EPHA1 (EAL23789.1) (Met1-Glu547) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Lys 26
• Form: Lyophilized from sterile PBS, pH 7.4, 5% ~ 8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized human EPHA1-His at 10 μg/ml (100 μl /well) can bind human EFNA1-Fch, The EC50 of human EFNA1-Fch is 30-70 ng/ml.
• Molecular Mass: The recombinant human EPHA1 consists 533 amino acids and predicts a molecular mass of 58 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPHA1 EPH receptor A1 [ Homo sapiens ]
• Official Symbol: EPHA1
• Synonyms: EPHA1; EPH receptor A1; EphA1 , EPHT, EPHT1; ephrin type-A receptor 1; EPH; hEpha1; oncogene EPH; eph tyrosine kinase 1; soluble EPHA1 variant 1; soluble EPHA1 variant 2; tyrosine-protein kinase receptor EPH; erythropoietin-producing hepatoma receptor; erythropoietin-producing hepatoma amplified sequence; EPHT; EPHT1; MGC163163
• Gene ID: 2041
• mRNA Refseq: NM_005232
• Protein Refseq: NP_005223
• MIM: 179610
• UniProt ID: P21709
• Chromosome Location: 7q32-q36
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; EPHA forward signaling, organism-specific biosystem