Recombinant Human EPO Protein, Fc-tagged

Cat.No.: EPO-200H
Product Overview: Recombinant human EPO (NP_000790.2) (Met1-Arg193) was expressed with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Human
Tag: Fc
Predicted N Terminal: Ala 28
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant human EPO consists 404 amino acids and predicts a molecular mass of 45.1 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: EPO erythropoietin [ Homo sapiens ]
Official Symbol: EPO
Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142;
Gene ID: 2056
mRNA Refseq: NM_000799
Protein Refseq: NP_000790
MIM: 133170
UniProt ID: P01588
Chromosome Location: 7q21
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; EPO Receptor Signaling, organism-specific biosystem; EPO signaling pathway, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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