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Home > Products > Recombinant Proteins > Recombinant Human EZH2 Complex protein, His/Flag-tagged

Recombinant Human EZH2 Complex protein, His/Flag-tagged

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Flag/His(1)
His(8)
His/Flag(15)
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Cat.No.:
EZH2-178H
Product Overview:
Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system.
Source:
Insect cells
Species:
Human
Tag:
His/Flag
Form:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Bio-activity:
0.08 pmol/min/µg
Molecular Mass:
325 kDa (complex)
Protein length:
2–end (all components)
Purity:
≥ 96%
Applications:
Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex. Requires EED protein for methyltransferase activity.
Storage:
>6 months at -80 centigrade. Avoid freeze/thaw cycles
Concentration:
0.31 mg/ml


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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