Recombinant Human EZH2 Complex protein fused with N-terminal His-tag (EZH2), N-terminal FLAG-tag (EED) was expressed in Insect cells.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 200 mM imidazole, and 20% glycerol.
138 kDa (complex)
2–end (EZH2 and EED)
Useful for Western blot analysis and to investigate protein-protein interactions in the EZH2 complex. Requires SUZ12 protein for methyltransferase activity.
>6 months at -80 centigrade. Avoid freeze/thaw cycles