Recombinant Human F2(Gln 25 - Glu 622) fused with His tag at C-terminal was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Has a calculated MW of 69.5 kDa. DTT-reduced Protein migrates as 85-110 kDa in SDS-PAGE due to glycosylation.
Gln 25 - Glu 622
Less than 1.0 EU per μg of the rh F2 / Thrombin by the LAL method.
>95% as determined by reduced SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade); After reconstitution under sterile conditions for 3 months (-70 centigrade).