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Active Recombinant Human F9 protein, Fc-tagged

Cat.No. : F9-258H
Product Overview : Recombinant Human F9(Thr29-Thr461) fused Fc tag was expressed in HEK293.
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Description : Coagulation Factor IX, also known as Christmas Factor, is a secreted by the liver and plays a key role in the activation of the intrinsic clotting cascade. Factor IX consists of a Gla domain, two tandem EGF-like domains, an activation peptide, and an S1 serine protease domain. Mature human Factor IX shares approximately 81% amino acid sequence identity with mouse and rat Factor IX. Alternative splicing generates an additional isoform that lacks the first EGF-like domain. The Gla domain is modified by Vitamin K-dependent gamma-carboxylation and mediates the association of Factor IX with phospholipid bilayers. The activation peptide is removed by Factor XIa mediated cleavage, resulting in heavy and light chains that remain disulfide-linked. Factor IX can also be activated by proteolytic factors in multiple snake venoms. Active Factor IX associates with Factor VIIIa on the platelet surface where it cleaves and activates Factor X, leading to Fibrin deposition and clot formation. The human Factor IX gene is highly polymorphic, and Hemophilia B can be caused by X-linked deficiency of Factor IX activity.
Source : HEK293
Species : Human
Tag : Fc
Predicted N Terminal : Thr29 (pro-mature) and Tyr47 (mature)
Form : Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Bio-activity : Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >30 pmol/min/μg.
Molecular Mass : Predicted Molecular Mass: 75 kDa
SDS-PAGE: 86-104 kDa, reducing conditions
Endotoxin : <0.1 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Gene Name : F9 coagulation factor IX [ Homo sapiens ]
Official Symbol : F9
Synonyms : F9; coagulation factor IX; Christmas disease; Factor IX; FIX; hemophilia B; plasma thromboplastic component; F9 p22; FIX F9; factor 9; factor IX F9; serine protease; Christmas factor; plasma thromboplastin component; P19; PTC; HEMB; THPH8; MGC129641; MGC129642;
Gene ID : 2158
mRNA Refseq : NM_000133
Protein Refseq : NP_000124
MIM : 300746
UniProt ID : P00740

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the function of F9 protein? 12/18/2019

The F9 protein is involved in step IX in the coagulation cascade, which works synergistically with other coagulation factors to promote the normal progress of blood clotting.

How do genetic mutations in the F9 protein affect its function? 12/04/2019

Mutations in the F9 gene may lead to loss of protein expression, structural abnormalities, or dysfunction, which in turn affect the normal function of the F9 protein.

Do F9 protein mutations cause other diseases or affect other physiological processes? 05/27/2019

F9 protein mutations are primarily associated with hemophilia B, but the specific mutation type and location may have different effects on an individual's clinical presentation.

What diseases can F9 gene mutations cause? 05/22/2019

Mutations in the F9 gene may cause hemophilia B, an inherited coagulation disorder in which patients lack the F9 protein or have low activity and are prone to abnormal bleeding.

How is the activity of F9 protein determined? 04/08/2019

The activity of the F9 protein is usually measured by coagulation time or by specific experimental methods (eg, prothrombin time, activated partial thromboplastin time, etc.).

What is the structure of F9 protein? 01/31/2019

F9 protein is a single-chain glycoprotein consisting of 415 amino acids. It contains a signaling peptide and multiple functional regions in which the Gla domain at the C-terminal binds to calcium ions and the EGF-like domain at the N-terminus participates in receptor binding and activation.

Customer Reviews (3)

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Reviews
04/15/2021

    The short half-life makes this product very safe.

    10/10/2020

      This protein has a wide range of applications and can be used in a variety of experiments.

      03/27/2019

        The method of use is simple, which reduces the experimental time and improves the efficiency.

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