Biotinylated Human Fc gamma RIIA / CD32a (H131) (CDA-H82E6) is expressed from human HEK293 cells. It contains AA Ala 36 - Ile 218 (Accession # P12318-1).
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay. Immobilized Human FcGR2A / CD32a (H131), His Tag can bind Ipilimumab with affinity constant of around 2.3 uM. Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (0.5 ug/well). The linear range is 0.5-16 ng/mL.
This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24 kDa. The protein migrates as 30-38 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.